6LPI

Crystal Structure of AHAS holo-enzyme

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (E, F, G, H)	Acetolactate synthase isozyme 1 small subunit	polymer	127	13640.3	4	UniProt (P0ADF8) Pfam (PF01842)	Escherichia coli (strain K12)	Acetohydroxy-acid synthase I small subunit,ALS-I
2	B, D, F, H (A, B, C, D)	Acetolactate synthase isozyme 1 large subunit	polymer	562	60496.3	4	UniProt (P08142) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775)	Escherichia coli (strain K12)	AHAS-I,Acetohydroxy-acid synthase I large subunit,ALS-I
3	I, L, O, R (A, B, C, D)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	4	Chemie (FAD)
4	J, M, P, S (A, B, C, D)	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
5	K, N, Q, T (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	U, V, W, X (F, G, C, D)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

E, F, G, H: 1 - 96 (UniProt: P0ADF8)

PDB	External Database	Details
Pro 0	-	expression tag
Ala 97	-	expression tag
Ser 98	-	expression tag
Thr 99	-	expression tag
Ala 100	-	expression tag
Ala 101	-	expression tag
Thr 102	-	expression tag
Ser 103	-	expression tag
Ala 104	-	expression tag
Ala 105	-	expression tag
Ala 106	-	expression tag
Thr 107	-	expression tag
Ser 108	-	expression tag
Ala 109	-	expression tag
Ala 110	-	expression tag
Ala 111	-	expression tag
Thr 112	-	expression tag
Ser 113	-	expression tag
Ala 114	-	expression tag
Ala 115	-	expression tag
Ala 116	-	expression tag
Thr 117	-	expression tag
Ser 118	-	expression tag
Ala 119	-	expression tag
Ala 120	-	expression tag
Ala 121	-	expression tag
Thr 122	-	expression tag
Ser 123	-	expression tag
Ala 124	-	expression tag
Ala 125	-	expression tag
Ser 126	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	296546.6
Non-Polymers*	Number of molecules	12
	Total formula weight	4940.7
All*	Total formula weight	301487.3

*Water molecules are not included.