6LML
Cryo-EM structure of the human glucagon receptor in complex with Gi1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 4 | D (D) | scFv16 | polymer | 247 | 26337.3 | 1 | Mus musculus (Mouse) | ||
| 5 | E (E) | Glucagon | polymer | 29 | 3486.8 | 1 | UniProt (P01275) Pfam (PF00123) | Homo sapiens | |
| 6 | F (R) | Glucagon receptor | polymer | 422 | 48571.4 | 1 | UniProt (P47871) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (Human) | GL-R |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
R: 27 - 432 (UniProt: P47871)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Arg 126 | Glu 126 | engineered mutation |
| Trp 200 | Thr 200 | engineered mutation |
| Met 366 | Ala 366 | engineered mutation |
| Gly 433 | - | expression tag |
| Ser 434 | - | expression tag |
| Gly 435 | - | expression tag |
| Ser 436 | - | expression tag |
| Glu 437 | - | expression tag |
| Asp 438 | - | expression tag |
| Gln 439 | - | expression tag |
| Val 440 | - | expression tag |
| Asp 441 | - | expression tag |
| Pro 442 | - | expression tag |
| Arg 443 | - | expression tag |
| Leu 444 | - | expression tag |
| Ile 445 | - | expression tag |
| Asp 446 | - | expression tag |
| Gly 447 | - | expression tag |
| Lys 448 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 165415.1 | |
| All* | Total formula weight | 165415.1 |
