6LI3
cryo-EM structure of GPR52-miniGs-NB35
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | R | G-protein coupled receptor 52 | polymer | 340 | 39128.8 | 1 | UniProt (Q9Y2T5) Pfam (PF00001) In PDB | Homo sapiens (Human) | |
2 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 248 | 28907.7 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein |
3 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
4 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7845.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
5 | N | nanobody Nb35 | polymer | 149 | 16054.2 | 1 | Lama glama |
Sequence modifications
R: 1 - 340 (UniProt: Q9Y2T5)
A: 6 - 64 (UniProt: P63092)
A: 204 - 394 (UniProt: P63092)
G: 1 - 71 (UniProt: P59768)
PDB | External Database | Details |
---|---|---|
Trp 130 | Ala 130 | engineered mutation |
Pro 314 | Cys 314 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Asp 49 | Gly 49 | engineered mutation |
Asn 50 | Glu 50 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 196 | - | linker |
Gly 197 | - | linker |
Ser 198 | - | linker |
Gly 199 | - | linker |
Gly 200 | - | linker |
Ser 201 | - | linker |
Gly 202 | - | linker |
Gly 203 | - | linker |
Asp 249 | Ala 249 | engineered mutation |
Asp 252 | Ser 252 | engineered mutation |
- | Asn 254 | deletion |
- | Met 255 | deletion |
- | Val 256 | deletion |
- | Ile 257 | deletion |
- | Arg 258 | deletion |
- | Glu 259 | deletion |
- | Asp 260 | deletion |
- | Asn 261 | deletion |
- | Gln 262 | deletion |
- | Thr 263 | deletion |
Ala 372 | Ile 372 | engineered mutation |
Ile 375 | Val 375 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ser 68 | Cys 68 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 129352.7 | |
All* | Total formula weight | 129352.7 |