6LI1

Crystal structure of GPR52 ligand free form with flavodoxin fusion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Chimera of G-protein coupled receptor 52 and Flavodoxin	polymer	449	50164.8	1	UniProt (Q9Y2T5) UniProt (P00323) Pfam (PF00001) Pfam (PF00258) In PDB	Homo sapiens (Human)
2	A	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	2	Chemie (OLC)
3	A	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
6		water	water		18.0	7	Chemie (HOH)

Sequence modifications

A: 17 - 236 (UniProt: Q9Y2T5)

PDB	External Database	Details
Gly 16	-	expression tag
Trp 130	Ala 130	engineered mutation

A: 1003 - 1148 (UniProt: P00323)

PDB	External Database	Details
Trp 1098	Tyr 98	engineered mutation
Arg 261	-	linker
Arg 262	-	linker
Tyr 263	-	linker
Leu 264	-	linker

A: 265 - 341 (UniProt: Q9Y2T5)

PDB	External Database	Details
Ala 1002	-	linker
Gln 278	Trp 278	engineered mutation
Pro 314	Cys 314	engineered mutation
Ala 318	Ser 318	engineered mutation
Asp 321	Asn 321	engineered mutation
Thr 323	Val 323	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50164.8
Non-Polymers*	Number of molecules	5
	Total formula weight	1425.7
All*	Total formula weight	51590.5

*Water molecules are not included.