6LGF

Bombyx mori GH13 sucrose hydrolase mutant D247N complexed with sucrose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Sucrose hydrolase	polymer	598	68738.2	2	UniProt (A0A077JI83) Pfam (PF00128)	Bombyx mori (Silk moth)
2	C, D (E, F)	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)
3	E, G, H, J (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	F, I (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	K, L (A, B)	water	water		18.0	566	Chemie (HOH)

Sequence modifications

A, B: 30 - 606 (UniProt: A0A077JI83)

PDB	External Database	Details
Met 9	-	expression tag
Gly 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
Ser 19	-	expression tag
Ser 20	-	expression tag
Gly 21	-	expression tag
Leu 22	-	expression tag
Val 23	-	expression tag
Pro 24	-	expression tag
Arg 25	-	expression tag
Gly 26	-	expression tag
Ser 27	-	expression tag
His 28	-	expression tag
Met 29	-	expression tag
Asn 247	Asp 247	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	137476.4
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	6
	Total formula weight	177.4
All*	Total formula weight	138338.4

*Water molecules are not included.