6LE2

Structure of D-carbamoylase mutant from Nitratireductor indicus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	N-carbamoyl-D-amino-acid hydrolase	polymer	316	35495.1	2	UniProt (K2NMS4) Pfam (PF00795)	Nitratireductor indicus C115
2	C, D, J, K (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
3	E, F, G, H, I... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	8	Chemie (EDO)
4	O, P (A, B)	water	water		18.0	210	Chemie (HOH)

Sequence modifications

A, B: 1 - 307 (UniProt: K2NMS4)

PDB	External Database	Details
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 187	Asp 187	engineered mutation
Asn 200	Ala 200	engineered mutation
Ala 207	Ser 207	engineered mutation
Gly 211	Arg 211	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70990.2
Non-Polymers*	Number of molecules	12
	Total formula weight	864.9
All*	Total formula weight	71855.1

*Water molecules are not included.