6LC5
Crystal structure of barley exohydrolaseI W434F in complex with 4'-nitrophenyl thiolaminaribioside
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Beta-D-glucan exohydrolase isoenzyme ExoI | polymer | 609 | 65855.0 | 1 | UniProt (Q9XEI3) Pfam (PF00933) Pfam (PF01915) In PDB | Hordeum vulgare subsp. vulgare (Domesticated barley) | |
| 2 | A | (2~{R},3~{S},4~{S},5~{R},6~{R})-6-(hydroxymethyl)-4-[(2~{S},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]sulfanyl-oxane-2,3,5-triol | non-polymer | 358.4 | 1 | Chemie (6BV) | |||
| 3 | A | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 11 | Chemie (1PE) | |||
| 4 | A | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 5 | A | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 6 | A | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 3 | Chemie (EPE) | |||
| 7 | A | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 8 | water | water | 18.0 | 502 | Chemie (HOH) |
Sequence modifications
A: 0 - 605 (UniProt: Q9XEI3)
| PDB | External Database | Details |
|---|---|---|
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Lys 320 | Asn 345 | conflict |
| Phe 434 | Trp 459 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 65855.0 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 4469.0 | |
| All* | Total formula weight | 70324.0 |
