6LBV
Crystal structure of barley exohydrolaseI W434F mutant in complex with methyl 6-thio-beta-gentiobioside
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Beta-D-glucan exohydrolase isoenzyme ExoI | polymer | 609 | 65855.0 | 1 | UniProt (Q9XEI3) Pfam (PF00933) Pfam (PF01915) | Hordeum vulgare subsp. vulgare (Domesticated barley) | |
| 2 | B (B) | beta-D-glucopyranose-(1-6)-methyl 6-thio-beta-D-glucopyranoside | branched | 372.4 | 1 | In PDB BIRD (PRD_900098) GlyTouCan (G14290QQ) | METHYL 6-THIO-BETA-GENTIOBIOSIDE | ||
| 3 | C, D, E, F, G... (A) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 4 | I, J, K (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 3 | Chemie (1PE) | |||
| 5 | L, M, N (A) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 6 | O (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 7 | P (A) | water | water | 18.0 | 601 | Chemie (HOH) |
Sequence modifications
A: 0 - 605 (UniProt: Q9XEI3)
| PDB | External Database | Details |
|---|---|---|
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Lys 320 | Asn 345 | See sequence details |
| Phe 434 | Trp 459 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 65855.0 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 372.4 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1614.6 | |
| All* | Total formula weight | 67842.1 |
