6L4W
Turning an asparaginyl endopeptidase into a peptide ligase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Asparaginyl endopeptidase | polymer | 502 | 55816.0 | 1 | UniProt (A0A0P0QM28) Pfam (PF01650) Pfam (PF20985) | Clitoria ternatea (Butterfly pea) | Butelase 2A |
| 2 | B (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 3 | C (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 4 | D (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 5 | E (A) | water | water | 18.0 | 392 | Chemie (HOH) |
Sequence modifications
A: 33 - 488 (UniProt: A0A0P0QM28)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | expression tag |
| Val -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ala -10 | - | expression tag |
| Ile -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Val -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ala 1 | - | expression tag |
| His 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Ala 4 | - | expression tag |
| Phe 5 | - | expression tag |
| Ala 6 | - | expression tag |
| Ala 7 | - | expression tag |
| Ala 8 | - | expression tag |
| Met 9 | - | expression tag |
| Gly 10 | - | expression tag |
| His 11 | - | expression tag |
| His 12 | - | expression tag |
| His 13 | - | expression tag |
| His 14 | - | expression tag |
| His 15 | - | expression tag |
| His 16 | - | expression tag |
| Ser 17 | - | expression tag |
| Ser 18 | - | expression tag |
| Gly 19 | - | expression tag |
| Val 20 | - | expression tag |
| Asp 21 | - | expression tag |
| Leu 22 | - | expression tag |
| Gly 23 | - | expression tag |
| Thr 24 | - | expression tag |
| Glu 25 | - | expression tag |
| Asn 26 | - | expression tag |
| Leu 27 | - | expression tag |
| Tyr 28 | - | expression tag |
| Phe 29 | - | expression tag |
| Gln 30 | - | expression tag |
| Ser 31 | - | expression tag |
| Met 32 | - | expression tag |
| Snn 179 | Asp 179 | modified residue |
| Val 252 | Gly 252 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 55816.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 389.4 | |
| All* | Total formula weight | 56205.3 |
