6KXN
Crystal structure of W50A mutant of Chitiniphilus shinanonensis chitinase ChiL (CsChiL) complexed with N,N'-diacetylchitobiose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Family 18 chitinase | polymer | 372 | 41272.8 | 2 | UniProt (F8WSX2) Pfam (PF00704) In PDB | Chitiniphilus shinanonensis | CsChiL |
| 2 | C, D | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 3 | A, B | SULFATE ION | non-polymer | 96.1 | 9 | Chemie (SO4) | |||
| 4 | B | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 5 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 7 | water | water | 18.0 | 761 | Chemie (HOH) |
Sequence modifications
A, B: 41 - 406 (UniProt: F8WSX2)
| PDB | External Database | Details |
|---|---|---|
| Gly 35 | - | expression tag |
| Gly 36 | - | expression tag |
| Ser 37 | - | expression tag |
| Gly 38 | - | expression tag |
| Gly 39 | - | expression tag |
| Ser 40 | - | expression tag |
| Ala 50 | Trp 50 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 82545.6 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 848.8 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1231.0 | |
| All* | Total formula weight | 84625.4 |
