6KQD

Thermus thermophilus initial transcription complex comprising sigma A and 5'-OH RNA of 3 nt

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, J, K (A, B, K, L)	DNA-directed RNA polymerase subunit alpha	polymer	315	35056.2	4	UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C, L (C, M)	DNA-directed RNA polymerase subunit beta	polymer	1119	125436.5	2	UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D, M (D, N)	DNA-directed RNA polymerase subunit beta'	polymer	1524	170997.4	2	UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E, N (E, O)	DNA-directed RNA polymerase subunit omega	polymer	99	11533.3	2	UniProt (Q8RQE7) Pfam (PF01192)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F, O (F, P)	RNA polymerase sigma factor SigA	polymer	443	50769.4	2	UniProt (Q5SKW1) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
6	G, P (G, Q)	DNA (5'-D(*CP*CP*T*GP*CP*AP*TP*CP*CP*GP*TP*GP*AP*GP*TP*CP*CP*AP*GP*GP*G)-3')	polymer	21	6440.1	2		synthetic construct
7	H, Q (H, R)	DNA (5'-D(*TP*AP*TP*AP*AP*TP*GP*GP*GP*AP*GP*CP*TP*GP*TP*CP*AP*CP*GP*GP*AP*TP*GP*CP*AP*GP*G*)-3')	polymer	27	8421.4	2		synthetic construct
8	I, R (I, S)	RNA (5'-R(*GP*GP*A)-3')	polymer	3	974.7	2		synthetic construct
9	CA, DA, S, V, W... (N, B, D, F, K)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
10	AA, BA, T, U (N, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
11	EA, Y (Q, G)	2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE	non-polymer		347.2	2	Chemie (DGP)
12	FA, GA, HA, IA, JA... (A, B, C, D, E...)	water	water		18.0	107	Chemie (HOH)

Sequence modifications

F, P: 1 - 423 (UniProt: Q5SKW1)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	889370.4
Non-Polymers*	Number of molecules	13
	Total formula weight	1126.2
All*	Total formula weight	890496.6

*Water molecules are not included.