6KPO

Crystal Structure of endo-beta-N-acetylglucosaminidase from Cordyceps militaris D154N/E156Q mutant in complex with fucosyl-N-acetylglucosamine-Asn

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Chitinase	polymer	303	33402.3	1	UniProt (G3JPF7) Pfam (PF00704) In PDB	Cordyceps militaris CM01 (Caterpillar fungus)
2	B	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G86851RC)
3	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
5	A	ASPARAGINE	non-polymer		132.1	1	Chemie (ASN)
6		water	water		18.0	115	Chemie (HOH)

Sequence modifications

A: 20 - 315 (UniProt: G3JPF7)

PDB	External Database	Details
Met 19	-	initiating methionine
Asn 154	Asp 154	engineered mutation
Gln 156	Glu 156	engineered mutation
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33402.3
Branched	Number of molecules	1
	Total formula weight	367.3
Non-Polymers*	Number of molecules	4
	Total formula weight	494.5
All*	Total formula weight	34264.2

*Water molecules are not included.