6KPO
Crystal Structure of endo-beta-N-acetylglucosaminidase from Cordyceps militaris D154N/E156Q mutant in complex with fucosyl-N-acetylglucosamine-Asn
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Chitinase | polymer | 303 | 33402.3 | 1 | UniProt (G3JPF7) Pfam (PF23916) | Cordyceps militaris CM01 (Caterpillar fungus) | |
| 2 | B (B) | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 367.3 | 1 | In PDB GlyTouCan (G86851RC) | |||
| 3 | C (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 4 | D, E (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 5 | F (A) | ASPARAGINE | non-polymer | 132.1 | 1 | Chemie (ASN) | |||
| 6 | G (A) | water | water | 18.0 | 115 | Chemie (HOH) |
Sequence modifications
A: 20 - 315 (UniProt: G3JPF7)
| PDB | External Database | Details |
|---|---|---|
| Met 19 | - | initiating methionine |
| Asn 154 | Asp 154 | engineered mutation |
| Gln 156 | Glu 156 | engineered mutation |
| His 316 | - | expression tag |
| His 317 | - | expression tag |
| His 318 | - | expression tag |
| His 319 | - | expression tag |
| His 320 | - | expression tag |
| His 321 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 33402.3 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 367.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 494.5 | |
| All* | Total formula weight | 34264.2 |
