6KPG
Cryo-EM structure of CB1-G protein complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 353 | 40283.8 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 346 | 38045.6 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
| 4 | R | Cannabinoid receptor 1 | polymer | 469 | 52843.7 | 1 | UniProt (P21554) Pfam (PF00001) In PDB | Homo sapiens (Human) | CB1,CANN6 |
| 5 | S | scFv16 | polymer | 259 | 27742.9 | 1 | Homo sapiens | ||
| 6 | R | (6~{a}~{R},9~{R},10~{a}~{R})-9-(hydroxymethyl)-3-(8-isothiocyanato-2-methyl-octan-2-yl)-6,6-dimethyl-6~{a},7,8,9,10,10~{a}-hexahydrobenzo[c]chromen-1-ol | non-polymer | 445.7 | 1 | Chemie (8D0) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: 71 - 425 (UniProt: P21554)
| PDB | External Database | Details |
|---|---|---|
| Ile -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Arg -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Phe 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala -43 | - | expression tag |
| Asp -42 | - | expression tag |
| Leu -41 | - | expression tag |
| Glu -40 | - | expression tag |
| Asp -39 | - | expression tag |
| Asn -38 | - | expression tag |
| Trp -37 | - | expression tag |
| Glu -36 | - | expression tag |
| Thr -35 | - | expression tag |
| Leu -34 | - | expression tag |
| Asn -33 | - | expression tag |
| Asp -32 | - | expression tag |
| Asn -31 | - | expression tag |
| Leu -30 | - | expression tag |
| Lys -29 | - | expression tag |
| Val -28 | - | expression tag |
| Ile -27 | - | expression tag |
| Glu -26 | - | expression tag |
| Lys -25 | - | expression tag |
| Ala -24 | - | expression tag |
| Asp -23 | - | expression tag |
| Asn -22 | - | expression tag |
| Ala -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Gln -19 | - | expression tag |
| Val -18 | - | expression tag |
| Lys -17 | - | expression tag |
| Asp -16 | - | expression tag |
| Ala -15 | - | expression tag |
| Leu -14 | - | expression tag |
| Thr -13 | - | expression tag |
| Lys -12 | - | expression tag |
| Met -11 | - | expression tag |
| Arg -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Ala -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Lys 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Glu 5 | - | expression tag |
| Asp 6 | - | expression tag |
| Lys 7 | - | expression tag |
| Ser 8 | - | expression tag |
| Pro 9 | - | expression tag |
| Asp 10 | - | expression tag |
| Ser 11 | - | expression tag |
| Pro 12 | - | expression tag |
| Glu 13 | - | expression tag |
| Met 14 | - | expression tag |
| Lys 15 | - | expression tag |
| Asp 16 | - | expression tag |
| Phe 17 | - | expression tag |
| Arg 18 | - | expression tag |
| His 19 | - | expression tag |
| Gly 20 | - | expression tag |
| Phe 21 | - | expression tag |
| Asp 22 | - | expression tag |
| Ile 23 | - | expression tag |
| Leu 24 | - | expression tag |
| Val 25 | - | expression tag |
| Gly 26 | - | expression tag |
| Gln 27 | - | expression tag |
| Ile 28 | - | expression tag |
| Asp 29 | - | expression tag |
| Asp 30 | - | expression tag |
| Ala 31 | - | expression tag |
| Leu 32 | - | expression tag |
| Lys 33 | - | expression tag |
| Leu 34 | - | expression tag |
| Ala 35 | - | expression tag |
| Asn 36 | - | expression tag |
| Glu 37 | - | expression tag |
| Gly 38 | - | expression tag |
| Lys 39 | - | expression tag |
| Val 40 | - | expression tag |
| Lys 41 | - | expression tag |
| Glu 42 | - | expression tag |
| Ala 43 | - | expression tag |
| Gln 44 | - | expression tag |
| Ala 45 | - | expression tag |
| Ala 46 | - | expression tag |
| Ala 47 | - | expression tag |
| Glu 48 | - | expression tag |
| Gln 49 | - | expression tag |
| Leu 50 | - | expression tag |
| Lys 51 | - | expression tag |
| Thr 52 | - | expression tag |
| Thr 53 | - | expression tag |
| Arg 54 | - | expression tag |
| Asn 55 | - | expression tag |
| Ala 56 | - | expression tag |
| Tyr 57 | - | expression tag |
| Ile 58 | - | expression tag |
| Gln 59 | - | expression tag |
| Lys 60 | - | expression tag |
| Tyr 61 | - | expression tag |
| Leu 62 | - | expression tag |
| Leu 63 | - | expression tag |
| Glu 64 | - | expression tag |
| Val 65 | - | expression tag |
| Leu 66 | - | expression tag |
| Phe 67 | - | expression tag |
| Gln 68 | - | expression tag |
| Gly 69 | - | expression tag |
| Pro 70 | - | expression tag |
| Lys 273 | Glu 273 | engineered mutation |
| Val 283 | Thr 283 | engineered mutation |
| Glu 340 | Arg 340 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 166777.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 445.7 | |
| All* | Total formula weight | 167222.8 |
