6KJ6

cryo-EM structure of Escherichia coli Crl transcription activation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli K-12	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli K-12	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1416	156537.0	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli (strain K12)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli K-12	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase sigma factor RpoS	polymer	334	38352.2	1	UniProt (P13445) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli (strain K12)	Sigma S,Sigma-38
6	G	DNA (51-MER)	polymer	51	15797.1	1		synthetic construct
7	H	DNA (51-MER)	polymer	51	15797.1	1		synthetic construct
8	I	RNA (5'-R(*CP*UP*CP*GP*A)-3')	polymer	5	1546.0	1		synthetic construct
9	J	Sigma factor-binding protein Crl	polymer	137	16009.3	1	UniProt (B1J0Z4) Pfam (PF07417) UniProt (by SIFTS) (P24251) In PDB	Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
10	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
11	I	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

D: 1 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Val 1410	-	expression tag
His 1411	-	expression tag
His 1412	-	expression tag
His 1413	-	expression tag
His 1414	-	expression tag
His 1415	-	expression tag
His 1416	-	expression tag

F: 1 - 330 (UniProt: P13445)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag

J: 1 - 133 (UniProt: B1J0Z4)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	478226.6
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	478381.7

*Water molecules are not included.