6KJ6
cryo-EM structure of Escherichia coli Crl transcription activation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1416 | 156537.0 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli (strain K12) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor RpoS | polymer | 334 | 38352.2 | 1 | UniProt (P13445) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli (strain K12) | Sigma S,Sigma-38 |
| 6 | G (G) | DNA (51-MER) | polymer | 51 | 15797.1 | 1 | synthetic construct | ||
| 7 | H (H) | DNA (51-MER) | polymer | 51 | 15797.1 | 1 | synthetic construct | ||
| 8 | I (I) | RNA (5'-R(*CP*UP*CP*GP*A)-3') | polymer | 5 | 1546.0 | 1 | synthetic construct | ||
| 9 | J (J) | Sigma factor-binding protein Crl | polymer | 137 | 16009.3 | 1 | UniProt (B1J0Z4) Pfam (PF07417) UniProt (by SIFTS) (P24251) | Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) | |
| 10 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 11 | M (I) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
D: 1 - 1407 (UniProt: P0A8T7)
F: 1 - 330 (UniProt: P13445)
J: 1 - 133 (UniProt: B1J0Z4)
| PDB | External Database | Details |
|---|---|---|
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Val 1410 | - | expression tag |
| His 1411 | - | expression tag |
| His 1412 | - | expression tag |
| His 1413 | - | expression tag |
| His 1414 | - | expression tag |
| His 1415 | - | expression tag |
| His 1416 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 478226.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 478381.7 |
