6KIX
Cryo-EM structure of human MLL1-NCP complex, binding mode1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3 | polymer | 135 | 15303.9 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) | Xenopus laevis (African clawed frog) | |
| 2 | B, F (B, F) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 3 | C, G (C, G) | Histone H2A | polymer | 129 | 13978.2 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B 1.1 | polymer | 122 | 13498.7 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I (I) | DNA (145-MER) | polymer | 145 | 44521.4 | 1 | synthetic construct | ||
| 6 | J (J) | DNA (145-MER) | polymer | 145 | 44992.6 | 1 | synthetic construct | ||
| 7 | K (N) | Retinoblastoma-binding protein 5 | polymer | 538 | 59223.5 | 1 | UniProt (Q15291) Pfam (PF00400) | Homo sapiens (Human) | RBBP-5,Retinoblastoma-binding protein RBQ-3 |
| 8 | L (K) | Histone-lysine N-methyltransferase 2A | polymer | 216 | 24970.5 | 1 | UniProt (Q03164) Pfam (PF00856) | Homo sapiens (Human) | Lysine N-methyltransferase 2A,ALL-1,CXXC-type zinc finger protein 7,Myeloid/lymphoid or mixed-lineage leukemia,Myeloid/lymphoid or mixed-lineage leukemia protein 1,Trithorax-like protein,Zinc finger protein HRX |
| 9 | M (R) | WD repeat-containing protein 5 | polymer | 334 | 36635.4 | 1 | UniProt (P61964) Pfam (PF25175) | Homo sapiens (Human) | BMP2-induced 3-kb gene protein |
| 10 | N (T) | Set1/Ash2 histone methyltransferase complex subunit ASH2 | polymer | 534 | 60288.8 | 1 | UniProt (Q9UBL3) Pfam (PF21257) Pfam (PF21198) Pfam (PF00622) | Homo sapiens (Human) | ASH2-like protein |
| 11 | O (K) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) PubChem (193) PubChem (13792) PubChem (189149) PubChem (439155) PubChem (5284331) PubChem (71170554) PubChem (13231890) PubChem (16760024) PubChem (25210495) PubChem (44361184) PubChem (177678424) PubChem (46936206) PubChem (21125603) PubChem (25203782) PubChem (52224400) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (6419189) PubChem (57544532) PubChem (59046843) PubChem (59170628) PubChem (164157219) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) | |||
| 12 | P (K) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 13 | Q (K) | LYSINE | non-polymer | 147.2 | 1 | Chemie (LYS) PubChem (4477687) PubChem (641599) | |||
| 14 | R (K) | GLUTAMINE | non-polymer | 146.1 | 1 | Chemie (GLN) PubChem (738) PubChem (5961) PubChem (145815) |
Sequence modifications
D, H: 1 - 122 (UniProt: P02281)
| PDB | External Database | Details |
|---|---|---|
| Thr 29 | Ser 33 | engineered mutation |
| Cys 117 | Lys 121 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 378720.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 743.2 | |
| All* | Total formula weight | 379463.6 |
