6KIU
Cryo-EM structure of human MLL1-ubNCP complex (3.2 angstrom)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3 | polymer | 135 | 15303.9 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) | Xenopus laevis (African clawed frog) | |
| 2 | B, F (B, F) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 3 | C, G (C, G) | Histone H2A | polymer | 129 | 13978.2 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B 1.1 | polymer | 122 | 13498.7 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I (I) | DNA (145-MER) | polymer | 145 | 44521.4 | 1 | synthetic construct | ||
| 6 | J (J) | DNA (145-MER) | polymer | 145 | 44992.6 | 1 | synthetic construct | ||
| 7 | K (K) | Histone-lysine N-methyltransferase 2A | polymer | 216 | 24970.5 | 1 | UniProt (Q03164) Pfam (PF00856) | Homo sapiens (Human) | Lysine N-methyltransferase 2A,ALL-1,CXXC-type zinc finger protein 7,Myeloid/lymphoid or mixed-lineage leukemia,Myeloid/lymphoid or mixed-lineage leukemia protein 1,Trithorax-like protein,Zinc finger protein HRX |
| 8 | L (N) | Retinoblastoma-binding protein 5 | polymer | 538 | 59223.5 | 1 | UniProt (Q15291) Pfam (PF00400) | Homo sapiens (Human) | RBBP-5,Retinoblastoma-binding protein RBQ-3 |
| 9 | M (R) | WD repeat-containing protein 5 | polymer | 334 | 36635.4 | 1 | UniProt (P61964) Pfam (PF25175) | Homo sapiens (Human) | BMP2-induced 3-kb gene protein |
| 10 | N (T) | Set1/Ash2 histone methyltransferase complex subunit ASH2 | polymer | 534 | 60288.8 | 1 | UniProt (Q9UBL3) Pfam (PF21257) Pfam (PF21198) Pfam (PF00622) | Homo sapiens (Human) | ASH2-like protein |
| 11 | O (O) | Ubiquitin | polymer | 76 | 8622.9 | 1 | UniProt (P62979) Pfam (PF00240) | Homo sapiens (Human) | |
| 12 | P (K) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (25210495) PubChem (13792) PubChem (177678424) PubChem (44361184) PubChem (21125603) PubChem (46936206) PubChem (50989525) PubChem (25203782) PubChem (51380914) PubChem (52224398) PubChem (189149) PubChem (57544532) PubChem (439155) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (52224400) PubChem (5284331) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (71170554) PubChem (13231890) PubChem (164157219) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) | |||
| 13 | Q (K) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 14 | R (K) | LYSINE | non-polymer | 147.2 | 1 | Chemie (LYS) PubChem (4477687) PubChem (641599) | |||
| 15 | S (K) | GLUTAMINE | non-polymer | 146.1 | 1 | Chemie (GLN) PubChem (738) PubChem (5961) PubChem (145815) |
Sequence modifications
D, H: 1 - 122 (UniProt: P02281)
O: 1 - 76 (UniProt: P62979)
| PDB | External Database | Details |
|---|---|---|
| Thr 29 | Ser 33 | engineered mutation |
| Cys 117 | Lys 121 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 76 | Gly 76 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 387343.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 743.2 | |
| All* | Total formula weight | 388086.5 |
