6KF3
Cryo-EM structure of Thermococcus kodakarensis RNA polymerase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | DNA-directed RNA polymerase subunit | polymer | 906 | 103038.6 | 1 | UniProt (Q5JE33) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 2 | B | DNA-directed RNA polymerase subunit beta | polymer | 1123 | 127468.0 | 1 | UniProt (Q5JE32) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 3 | C | DNA-directed RNA polymerase subunit A'' | polymer | 391 | 43727.4 | 1 | UniProt (Q5JE34) Pfam (PF04998) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 4 | D | DNA-directed RNA polymerase subunit D | polymer | 259 | 29429.6 | 1 | UniProt (Q5JJF4) Pfam (PF01193) Pfam (PF01000) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 5 | E | DNA-directed RNA polymerase, subunit E | polymer | 190 | 21893.4 | 1 | UniProt (Q5JIY4) Pfam (PF03876) Pfam (PF00575) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 6 | F | DNA-directed RNA polymerase, subunit F | polymer | 122 | 14519.7 | 1 | UniProt (Q5JI52) Pfam (PF03874) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 7 | H | DNA-directed RNA polymerase subunit H | polymer | 82 | 9522.0 | 1 | UniProt (Q5JE31) Pfam (PF01191) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 8 | K | DNA-directed RNA polymerase subunit K | polymer | 57 | 6286.5 | 1 | UniProt (Q5JJD0) Pfam (PF01192) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 9 | L | DNA-directed RNA polymerase subunit L | polymer | 94 | 11013.5 | 1 | UniProt (Q5JE88) Pfam (PF13656) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 10 | N | DNA-directed RNA polymerase subunit N | polymer | 65 | 7602.0 | 1 | UniProt (Q5JJC9) Pfam (PF01194) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 11 | P | DNA-directed RNA polymerase subunit P | polymer | 49 | 5553.7 | 1 | UniProt (Q5JDM8) Pfam (PF03604) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 12 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 13 | A, B, N, P | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) |
Sequence modifications
F: 1 - 114 (UniProt: Q5JI52)
| PDB | External Database | Details |
|---|---|---|
| Ile 115 | - | expression tag |
| Asp 116 | - | expression tag |
| Glu 117 | - | expression tag |
| Tyr 118 | - | expression tag |
| Arg 119 | - | expression tag |
| Pro 120 | - | expression tag |
| Leu 121 | - | expression tag |
| Glu 122 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 380054.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 351.4 | |
| All* | Total formula weight | 380405.9 |
