6KC8
Crystal structure of WT Nme1Cas9 in complex with sgRNA and target DNA (ATATGATT PAM) in post-cleavage state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1083 | 124701.0 | 1 | UniProt (C9X1G5) | Neisseria meningitidis 8013 | |
| 2 | B (B) | sgRNA | polymer | 135 | 43059.4 | 1 | synthetic construct | ||
| 3 | C (C) | DNA (5'-D(P*AP*AP*GP*TP*TP*AP*AP*AP*TP*AP*GP*CP*AP*GP*AP*GP*TP*GP*AP*CP*C)-3') | polymer | 21 | 6504.3 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(*AP*TP*AP*TP*GP*AP*TP*TP*TP*TP*A)-3') | polymer | 11 | 3362.2 | 1 | synthetic construct | ||
| 5 | E (P) | DNA (5'-D(*TP*AP*AP*AP*AP*TP*CP*AP*TP*AP*TP*GP*TP*A)-3') | polymer | 14 | 4286.8 | 1 | synthetic construct | ||
| 6 | F, G, H (A, B, C) | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
A: 1 - 1082 (UniProt: C9X1G5)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 181913.6 | |
| All* | Total formula weight | 181913.6 |
