6K83

Structure of RGLG1 mutant-D207G

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A, C, D, E...	E3 ubiquitin-protein ligase RGLG1	polymer	301	33107.1	8	UniProt (Q9SS90) Pfam (PF07002) In PDB	Arabidopsis thaliana (Mouse-ear cress)	RING domain ligase 1
2	F, G, B, H, A...	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
3	E, F, G, B, H...	CALCIUM ION	non-polymer		40.1	24	Chemie (CA)
4		water	water		18.0	505	Chemie (HOH)

Sequence modifications

B, A, C, D, E, F, G, H: 129 - 419 (UniProt: Q9SS90)

PDB	External Database	Details
Gly 119	-	expression tag
Thr 120	-	expression tag
Ser 121	-	expression tag
Ser 122	-	expression tag
Met 123	-	expression tag
Ala 124	-	expression tag
Asp 125	-	expression tag
Ile 126	-	expression tag
Gly 127	-	expression tag
Ser 128	-	expression tag
Gly 207	Asp 207	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	264856.8
Non-Polymers*	Number of molecules	32
	Total formula weight	1156.3
All*	Total formula weight	266013.1

*Water molecules are not included.