6K4U
Crystal structure of xCas9 in complex with sgRNA and DNA (TGA PAM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | CRISPR-associated endonuclease Cas9 | polymer | 1368 | 158538.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes serotype M1 | SpCas9,SpyCas9 |
| 2 | B (A) | sgRNA | polymer | 83 | 26789.0 | 1 | Streptococcus pyogenes | ||
| 3 | C (C) | target DNA | polymer | 28 | 8567.6 | 1 | Streptococcus pyogenes | ||
| 4 | D (D) | non-target DNA | polymer | 12 | 3709.5 | 1 | Streptococcus pyogenes |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Thr 262 | Ala 262 | engineered mutation |
| Leu 324 | Arg 324 | engineered mutation |
| Ile 409 | Ser 409 | engineered mutation |
| Lys 480 | Glu 480 | engineered mutation |
| Asp 543 | Glu 543 | engineered mutation |
| Ile 694 | Met 694 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
| Val 1219 | Glu 1219 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 197604.9 | |
| All* | Total formula weight | 197604.9 |
