6K08

Crystal structure of REV7(R124A/A135D) in complex with a Shieldin3 fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mitotic spindle assembly checkpoint protein MAD2B	polymer	219	25239.2	1	UniProt (Q9UI95) Pfam (PF02301)	Homo sapiens (Human)	Mitotic arrest deficient 2-like protein 2,MAD2-like protein 2,REV7 homolog,hREV7
2	B (B)	Shieldin complex subunit 3	polymer	30	3358.9	1	UniProt (Q6ZNX1)	Homo sapiens (Human)	REV7-interacting novel NHEJ regulator 1,Shield complex subunit 3
3	C (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	D, E (A, B)	water	water		18.0	14	Chemie (HOH)

Sequence modifications

A: 7 - 211 (UniProt: Q9UI95)

PDB	External Database	Details
Met -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
Ser 3	-	expression tag
Gln 4	-	expression tag
Asp 5	-	expression tag
Pro 6	-	expression tag
Ala 124	Arg 124	engineered mutation
Asp 135	Ala 135	engineered mutation

B: 46 - 74 (UniProt: Q6ZNX1)

PDB	External Database	Details
Met 45	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	28598.1
Non-Polymers*	Number of molecules	1
	Total formula weight	96.1
All*	Total formula weight	28694.2

*Water molecules are not included.