6JUK
Crystal structure of Formate dehydrogenase mutant C256I/E261P/S381I from Pseudomonas sp. 101 in complex with non-natural cofactor Nicotinamide Cytosine Dinucleotide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Formate dehydrogenase | polymer | 401 | 44198.2 | 2 | UniProt (P33160) Pfam (PF00389) Pfam (PF02826) | Pseudomonas sp. 101 (Achromobacter parvulus T1) | FDH,NAD-dependent formate dehydrogenase |
| 2 | C, D, E, F, I... (A, B) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 3 | G, H (A, B) | [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate | non-polymer | 640.4 | 2 | Chemie (A7R) | |||
| 4 | K, L (A, B) | water | water | 18.0 | 401 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 401 (UniProt: P33160)
| PDB | External Database | Details |
|---|---|---|
| Ile 256 | Cys 256 | engineered mutation |
| Pro 261 | Glu 261 | engineered mutation |
| Ile 381 | Ser 381 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 88396.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1833.4 | |
| All* | Total formula weight | 90229.7 |
