6JU1

p-Hydroxybenzoate hydroxylase Y385F mutant complexed with 3,4-dihydroxybenzoate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	4-hydroxybenzoate 3-monooxygenase	polymer	414	46537.9	1	UniProt (A0A367MFY2) Pfam (PF01494) UniProt (by SIFTS) (P20586)	Pseudomonas aeruginosa
2	B (A)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
3	C (A)	3,4-DIHYDROXYBENZOIC ACID	non-polymer		154.1	1	Chemie (DHB)
4	D, E, F, G (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	H (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
6	I (A)	3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL	non-polymer		326.4	1	Chemie (P33)
7	J, K (A)	BROMIDE ION	non-polymer		79.9	2	Chemie (BR)
8	L (A)	water	water		18.0	398	Chemie (HOH)

Sequence modifications

A: 1 - 394 (UniProt: A0A367MFY2)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Phe 385	Tyr 385	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46537.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1988.5
All*	Total formula weight	48526.4

*Water molecules are not included.