6JSU

Structure of Geobacillus kaustophilus lactonase, Y99C/D266N double mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Phosphotriesterase	polymer	330	36818.9	2	UniProt (Q5KZU5) Pfam (PF02126) In PDB	Geobacillus kaustophilus (strain HTA426)
2	A, B	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A, B	HYDROXIDE ION	non-polymer		17.0	2	Chemie (OH)
5		water	water		18.0	467	Chemie (HOH)

Sequence modifications

A, B: 1 - 326 (UniProt: Q5KZU5)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Asn 0	-	expression tag
Cys 99	Tyr 99	engineered mutation
Asn 266	Asp 266	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73637.8
Non-Polymers*	Number of molecules	6
	Total formula weight	276.5
All*	Total formula weight	73914.3

*Water molecules are not included.