6JST
Structure of Geobacillus kaustophilus lactonase, Y99P/D266N double mutant with bound 3-oxo-C8-HSL
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Phosphotriesterase | polymer | 330 | 36812.9 | 4 | UniProt (Q5KZU5) Pfam (PF02126) | Geobacillus kaustophilus (strain HTA426) | |
| 2 | E, I, L, O (A, B, C, D) | FE (III) ION | non-polymer | 55.8 | 4 | Chemie (FE) | |||
| 3 | F, J, M, P (A, B, C, D) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 4 | G, K, N (A, B, C) | HYDROXIDE ION | non-polymer | 17.0 | 3 | Chemie (OH) | |||
| 5 | H (A) | 3-OXO-OCTANOIC ACID (2-OXO-TETRAHYDRO-FURAN-3-YL)-AMIDE | non-polymer | 241.3 | 1 | Chemie (LAE) | |||
| 6 | Q, R, S, T (A, B, C, D) | water | water | 18.0 | 762 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 326 (UniProt: Q5KZU5)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Pro 99 | Tyr 99 | engineered mutation |
| Asn 266 | Asp 266 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 147251.5 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 777.3 | |
| All* | Total formula weight | 148028.9 |
