6JPL

The X-ray structure of yeast tRNA methyltransferase Trm7-Trm734 in complex with S-adenosyl-L-methionine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	tRNA (guanosine(34)-2'-O)-methyltransferase non-catalytic subunit TRM734	polymer	1028	116391.8	2	UniProt (Q08924) Pfam (PF00400) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)
2	B, D	tRNA (cytidine(34)/guanosine(34)-2'-O)-methyltransferase	polymer	325	36546.5	2	UniProt (P38238) Pfam (PF01728) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)
3	A, C	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)
4	A, C	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
5	B, D	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
6		water	water		18.0	461	Chemie (HOH)

Sequence modifications

A, C: 1 - 1013 (UniProt: Q08924)

PDB	External Database	Details
Leu 1014	-	expression tag
Glu 1015	-	expression tag
Val 1016	-	expression tag
Leu 1017	-	expression tag
Phe 1018	-	expression tag
Gln 1019	-	expression tag
Gly 1020	-	expression tag
Pro 1021	-	expression tag
Ser 1022	-	expression tag
His 1023	-	expression tag
His 1024	-	expression tag
His 1025	-	expression tag
His 1026	-	expression tag
His 1027	-	expression tag
His 1028	-	expression tag

B, D: 1 - 310 (UniProt: P38238)

PDB	External Database	Details
Leu 311	-	expression tag
Glu 312	-	expression tag
Val 313	-	expression tag
Leu 314	-	expression tag
Phe 315	-	expression tag
Gln 316	-	expression tag
Gly 317	-	expression tag
Pro 318	-	expression tag
Ser 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	305876.6
Non-Polymers*	Number of molecules	14
	Total formula weight	2234.1
All*	Total formula weight	308110.7

*Water molecules are not included.