6JCX
Mycobacterium tuberculosis transcription initiation complex with ECF sigma factor sigma H and 6nt RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 368 | 40279.0 | 2 | UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Mycobacterium tuberculosis H37Rv | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1174 | 129602.3 | 1 | UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Mycobacterium tuberculosis H37Rv | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1317 | 147068.1 | 1 | UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Mycobacterium tuberculosis H37Rv | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 110 | 11851.1 | 1 | UniProt (P9WGY5) Pfam (PF01192) | Mycobacterium tuberculosis H37Rv | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | ECF RNA polymerase sigma factor SigH | polymer | 218 | 24382.2 | 1 | UniProt (P9WGH9) Pfam (PF04542) Pfam (PF08281) | Mycobacterium tuberculosis H37Rv | ECF sigma factor SigH,Alternative RNA polymerase sigma factor SigH,RNA polymerase sigma-H factor,Sigma-H factor |
| 6 | G (H) | DNA (5'-D(*TP*GP*CP*AP*TP*CP*CP*GP*TP*GP*AP*GP*TP*CP*GP*AP*GP*GP*GP*T)-3') | polymer | 20 | 6206.0 | 1 | synthetic construct | ||
| 7 | H (G) | DNA (5'-D(*TP*TP*GP*TP*GP*GP*GP*AP*GP*CP*TP*GP*TP*CP*AP*CP*GP*GP*AP*TP*GP*CP*A)-3') | polymer | 23 | 7152.6 | 1 | synthetic construct | ||
| 8 | I (I) | RNA (5'-R(*CP*CP*UP*CP*GP*A)-3') | polymer | 6 | 1851.2 | 1 | synthetic construct | ||
| 9 | J, K (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | L (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 11 | M, N, O, P, Q... (A, B, C, D, E...) | water | water | 18.0 | 185 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 347 (UniProt: P9WGZ1)
C: 7 - 1178 (UniProt: P9WGY9)
D: 2 - 1316 (UniProt: P9WGY7)
F: 1 - 216 (UniProt: P9WGH9)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | initiating methionine |
| Gly -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Ser -7 | - | expression tag |
| Gly -6 | - | expression tag |
| His -5 | - | expression tag |
| Ile -4 | - | expression tag |
| Glu -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Arg -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 5 | - | initiating methionine |
| Val 6 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Val 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 408671.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 408826.7 |
