6JBQ
CryoEM structure of Escherichia coli sigmaE transcription initiation complex containing 5nt of RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli (strain K12) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli (strain K12) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1416 | 156537.0 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli (strain K12) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli (strain K12) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F | ECF RNA polymerase sigma-E factor | polymer | 219 | 25113.4 | 1 | UniProt (P0AGB6) Pfam (PF04542) Pfam (PF08281) In PDB | Escherichia coli (strain K12) | RNA polymerase sigma-E factor,Sigma-24 |
| 6 | G | DNA (48-MER) | polymer | 48 | 14823.5 | 1 | synthetic construct | ||
| 7 | H | DNA (48-MER) | polymer | 48 | 14797.5 | 1 | synthetic construct | ||
| 8 | I | RNA (5'-R(*CP*UP*CP*GP*A)-3') | polymer | 5 | 1546.0 | 1 | synthetic construct | ||
| 9 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
D: 1 - 1407 (UniProt: P0A8T7)
F: 1 - 191 (UniProt: P0AGB6)
| PDB | External Database | Details |
|---|---|---|
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Val 1410 | - | expression tag |
| His 1411 | - | expression tag |
| His 1412 | - | expression tag |
| His 1413 | - | expression tag |
| His 1414 | - | expression tag |
| His 1415 | - | expression tag |
| His 1416 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -27 | - | expression tag |
| Ser -26 | - | expression tag |
| Ser -25 | - | expression tag |
| Tyr -24 | - | expression tag |
| Tyr -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| Asp -16 | - | expression tag |
| Tyr -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Ile -13 | - | expression tag |
| Pro -12 | - | expression tag |
| Thr -11 | - | expression tag |
| Thr -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Asn -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Phe -5 | - | expression tag |
| Gln -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 447005.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 447160.3 |
