6J9F

Cryo-EM structure of Xanthomonos oryzae transcription elongation complex with the bacteriophage protein P7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	346	38030.2	2	UniProt (B2SQT4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Xanthomonas oryzae pv. oryzae PXO99A	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1383	154471.7	1	UniProt (Q2NZX8) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) In PDB	Xanthomonas oryzae pv. oryzae MAFF 311018	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1405	155444.6	1	UniProt (B2SQQ2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Xanthomonas oryzae pv. oryzae PXO99A	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	99	11112.5	1	UniProt (A0A0U4VN94) Pfam (PF01192) UniProt (by SIFTS) (Q5H3R9) In PDB	Xanthomonas oryzae pv. oryzae	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	G	DNA (29-MER)	polymer	29	8813.6	1		synthetic construct
6	H	DNA (29-MER)	polymer	29	8840.7	1		synthetic construct
7	I	RNA (5'-R(P*AP*GP*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3')	polymer	20	6510.0	1		synthetic construct
8	J	45L	polymer	77	8519.4	1	UniProt (Q8LTJ5) Pfam (PF20800) In PDB	Xanthomonas virus Xp10	RNA polymerase inhibitor p7
9	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
10	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: 1 - 332 (UniProt: B2SQT4)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

J: 1 - 73 (UniProt: Q8LTJ5)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	429772.9
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	429928.0

*Water molecules are not included.