6J9F
Cryo-EM structure of Xanthomonos oryzae transcription elongation complex with the bacteriophage protein P7
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 346 | 38030.2 | 2 | UniProt (B2SQT4) | Xanthomonas oryzae pv. oryzae PXO99A | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1383 | 154471.7 | 1 | UniProt (Q2NZX8) | Xanthomonas oryzae pv. oryzae MAFF 311018 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1405 | 155444.6 | 1 | UniProt (B2SQQ2) | Xanthomonas oryzae pv. oryzae PXO99A | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 99 | 11112.5 | 1 | UniProt (A0A0U4VN94) Pfam (PF01192) UniProt (by SIFTS) (Q5H3R9) | Xanthomonas oryzae pv. oryzae | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (G) | DNA (29-MER) | polymer | 29 | 8813.6 | 1 | synthetic construct | ||
| 6 | G (H) | DNA (29-MER) | polymer | 29 | 8840.7 | 1 | synthetic construct | ||
| 7 | H (I) | RNA (5'-R(P*AP*GP*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3') | polymer | 20 | 6510.0 | 1 | synthetic construct | ||
| 8 | I (J) | 45L | polymer | 77 | 8519.4 | 1 | UniProt (Q8LTJ5) | Xanthomonas virus Xp10 | RNA polymerase inhibitor p7 |
| 9 | J (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 332 (UniProt: B2SQT4)
J: 1 - 73 (UniProt: Q8LTJ5)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 429772.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 429928.0 |
