6J99
Cryo-EM structure of human DOT1L in complex with an H2B-monoubiquitinated nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3 | polymer | 136 | 15435.1 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 2 | B, F (B, F) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 3 | C, G (C, G) | Histone H2A | polymer | 130 | 14109.4 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B 1.1 | polymer | 123 | 13629.9 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I (I) | DNA (144-MER) | polymer | 147 | 45138.8 | 1 | synthetic construct | ||
| 6 | J (J) | DNA (145-MER) | polymer | 147 | 45610.0 | 1 | synthetic construct | ||
| 7 | K (L) | Ubiquitin | polymer | 76 | 8622.9 | 1 | UniProt (P62979) Pfam (PF00240) | Homo sapiens (Human) | |
| 8 | L (K) | Histone-lysine N-methyltransferase, H3 lysine-79 specific | polymer | 416 | 47506.2 | 1 | UniProt (Q8TEK3) Pfam (PF08123) | Homo sapiens (Human) | DOT1-like protein,Histone H3-K79 methyltransferase,H3-K79-HMTase,Lysine N-methyltransferase 4 |
| 9 | M (K) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (25210495) PubChem (13792) PubChem (177678424) PubChem (44361184) PubChem (21125603) PubChem (46936206) PubChem (50989525) PubChem (25203782) PubChem (51380914) PubChem (52224398) PubChem (189149) PubChem (57544532) PubChem (439155) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (52224400) PubChem (5284331) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (71170554) PubChem (13231890) PubChem (164157219) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) |
Sequence modifications
D, H: 1 - 122 (UniProt: P02281)
L: 1 - 76 (UniProt: P62979)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Thr 29 | Ser 33 | engineered mutation |
| Cys 117 | Lys 121 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 76 | Gly 76 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 256015.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 384.4 | |
| All* | Total formula weight | 256400.1 |
