6J6N
Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 2 | B (C) | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114174.0 | 1 | UniProt (P36048) Pfam (PF03764) Pfam (PF00679) Pfam (PF16004) Pfam (PF00009) | Saccharomyces cerevisiae S288c (Baker's yeast) | 114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10 |
| 3 | C (J) | Pre-mRNA-splicing factor CWC21 | polymer | 135 | 15793.6 | 1 | UniProt (Q03375) Pfam (PF08312) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 4 | D (O) | Pre-mRNA-splicing factor PRP46 | polymer | 451 | 50771.3 | 1 | UniProt (Q12417) Pfam (PF00400) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 5 | E (P) | Pre-mRNA-processing protein 45 | polymer | 379 | 42548.7 | 1 | UniProt (P28004) Pfam (PF02731) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 6 | F (Q) | Pre-mRNA-splicing factor SLT11 | polymer | 364 | 40988.6 | 1 | UniProt (P38241) Pfam (PF21369) | Saccharomyces cerevisiae S288c (Baker's yeast) | Extracellular mutant protein 2 |
| 7 | G (R) | Pre-mRNA-splicing factor CWC2 | polymer | 339 | 38486.6 | 1 | UniProt (Q12046) Pfam (PF16131) Pfam (PF00076) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 8 | H (S) | Pre-mRNA-splicing factor CWC15 | polymer | 175 | 19975.2 | 1 | UniProt (Q03772) Pfam (PF04889) | Saccharomyces cerevisiae S288c (Baker's yeast) | Complexed with CEF1 protein 15 |
| 9 | I (T) | Pre-mRNA-splicing factor BUD31 | polymer | 157 | 18484.5 | 1 | UniProt (P25337) Pfam (PF01125) | Saccharomyces cerevisiae S288c (Baker's yeast) | Bud site selection protein 31 |
| 10 | J (Z) | Pre-mRNA-splicing factor CWC22 | polymer | 577 | 67386.1 | 1 | UniProt (P53333) Pfam (PF02847) | Saccharomyces cerevisiae S288c (Baker's yeast) | Complexed with CEF1 protein 22 |
| 11 | K (c) | Pre-mRNA-splicing factor CEF1 | polymer | 590 | 67837.8 | 1 | UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 12 | L (d) | Pre-mRNA-splicing factor CLF1 | polymer | 687 | 82555.9 | 1 | UniProt (Q12309) Pfam (PF23233) Pfam (PF23240) | Saccharomyces cerevisiae S288c (Baker's yeast) | Crooked neck-like factor 1,PRP19-associated complex protein 77,Synthetic lethal with CDC40 protein 3 |
| 13 | M (I) | Pre-mRNA-splicing factor SYF2 | polymer | 215 | 24850.7 | 1 | UniProt (P53277) Pfam (PF08231) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 14 | N (n) | Pre-mRNA-processing factor 17 | polymer | 455 | 52208.7 | 1 | UniProt (P40968) Pfam (PF00400) | Saccharomyces cerevisiae S288c (Baker's yeast) | Cell division control protein 40 |
| 15 | O (H) | Pre-mRNA-splicing factor ISY1 | polymer | 235 | 28073.8 | 1 | UniProt (P21374) Pfam (PF06246) | Saccharomyces cerevisiae S288c (Baker's yeast) | Interactor of SYF1 |
| 16 | P (B) | UBC4 pre-mRNA | polymer | 246 | 78814.5 | 1 | Saccharomyces cerevisiae S288c | ||
| 17 | Q (D) | U5 snRNA | polymer | 214 | 68643.3 | 1 | Saccharomyces cerevisiae S288c | ||
| 18 | R (E) | U6 snRNA | polymer | 112 | 35883.2 | 1 | Saccharomyces cerevisiae S288c | ||
| 19 | S (L) | U2 snRNA | polymer | 1175 | 376267.4 | 1 | Saccharomyces cerevisiae S288c | ||
| 20 | T (v) | Pre-mRNA-splicing factor SYF1 | polymer | 859 | 100344.0 | 1 | UniProt (Q04048) Pfam (PF23233) Pfam (PF23220) Pfam (PF23231) | Saccharomyces cerevisiae S288c (Baker's yeast) | PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1 |
| 21 | U (a) | U2 small nuclear ribonucleoprotein B'' | polymer | 111 | 12850.9 | 1 | UniProt (P40567) Pfam (PF13893) | Saccharomyces cerevisiae S288c (Baker's yeast) | U2 snRNP B'' |
| 22 | V (b) | U2 small nuclear ribonucleoprotein A' | polymer | 238 | 27232.3 | 1 | UniProt (Q08963) Pfam (PF14580) | Saccharomyces cerevisiae S288c (Baker's yeast) | U2 snRNP A' |
| 23 | W (t) | Pre-mRNA-splicing factor SNT309 | polymer | 175 | 20741.5 | 1 | UniProt (Q06091) | Saccharomyces cerevisiae S288c (Baker's yeast) | Synergistic to PRP19 mutation protein 309 |
| 24 | GA, X (u, i) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-E |
| 25 | KA, Y (m, z) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D1 |
| 26 | IA, Z (j, x) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-G |
| 27 | AA, HA (h, w) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-F |
| 28 | BA, DA (g, e) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D2 |
| 29 | CA, EA (k, s) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-B |
| 30 | FA, JA (l, y) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D3 |
| 31 | LA, MA, NA, OA (r, p, q, o) | Pre-mRNA-processing factor 19 | polymer | 503 | 56629.8 | 4 | UniProt (P32523) Pfam (PF08606) | Saccharomyces cerevisiae S288c (Baker's yeast) | RING-type E3 ubiquitin transferase PRP19 |
| 32 | PA (A) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
| 33 | QA (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 34 | AB, BB, CB, RA, YA... (E, C) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 35 | SA, TA, UA, VA, WA... (Q, R, T) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 41 |
| Total formula weight | 2074070.8 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1721.5 | |
| All* | Total formula weight | 2075792.3 |
