6J6N

Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pre-mRNA-splicing factor 8	polymer	2413	279867.5	1	UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)
2	C	Pre-mRNA-splicing factor SNU114	polymer	1008	114174.0	1	UniProt (P36048) Pfam (PF03764) Pfam (PF00679) Pfam (PF16004) Pfam (PF00009) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10
3	J	Pre-mRNA-splicing factor CWC21	polymer	135	15793.6	1	UniProt (Q03375) Pfam (PF08312) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)
4	O	Pre-mRNA-splicing factor PRP46	polymer	451	50771.3	1	UniProt (Q12417) Pfam (PF00400) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)
5	P	Pre-mRNA-processing protein 45	polymer	379	42548.7	1	UniProt (P28004) Pfam (PF02731) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)
6	Q	Pre-mRNA-splicing factor SLT11	polymer	364	40988.6	1	UniProt (P38241) Pfam (PF21369) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Extracellular mutant protein 2
7	R	Pre-mRNA-splicing factor CWC2	polymer	339	38486.6	1	UniProt (Q12046) Pfam (PF16131) Pfam (PF00076) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)
8	S	Pre-mRNA-splicing factor CWC15	polymer	175	19975.2	1	UniProt (Q03772) Pfam (PF04889) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Complexed with CEF1 protein 15
9	T	Pre-mRNA-splicing factor BUD31	polymer	157	18484.5	1	UniProt (P25337) Pfam (PF01125) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Bud site selection protein 31
10	Z	Pre-mRNA-splicing factor CWC22	polymer	577	67386.1	1	UniProt (P53333) Pfam (PF02847) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Complexed with CEF1 protein 22
11	c	Pre-mRNA-splicing factor CEF1	polymer	590	67837.8	1	UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)
12	d	Pre-mRNA-splicing factor CLF1	polymer	687	82555.9	1	UniProt (Q12309) Pfam (PF02184) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Crooked neck-like factor 1,PRP19-associated complex protein 77,Synthetic lethal with CDC40 protein 3
13	I	Pre-mRNA-splicing factor SYF2	polymer	215	24850.7	1	UniProt (P53277) Pfam (PF08231) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)
14	n	Pre-mRNA-processing factor 17	polymer	455	52208.7	1	UniProt (P40968) Pfam (PF00400) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Cell division control protein 40
15	H	Pre-mRNA-splicing factor ISY1	polymer	235	28073.8	1	UniProt (P21374) Pfam (PF06246) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Interactor of SYF1
16	B	UBC4 pre-mRNA	polymer	246	78814.5	1		Saccharomyces cerevisiae S288c
17	D	U5 snRNA	polymer	214	68643.3	1		Saccharomyces cerevisiae S288c
18	E	U6 snRNA	polymer	112	35883.2	1		Saccharomyces cerevisiae S288c
19	L	U2 snRNA	polymer	1175	376267.4	1		Saccharomyces cerevisiae S288c
20	v	Pre-mRNA-splicing factor SYF1	polymer	859	100344.0	1	UniProt (Q04048) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1
21	a	U2 small nuclear ribonucleoprotein B''	polymer	111	12850.9	1	UniProt (P40567) Pfam (PF13893) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	U2 snRNP B''
22	b	U2 small nuclear ribonucleoprotein A'	polymer	238	27232.3	1	UniProt (Q08963) Pfam (PF14580) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	U2 snRNP A'
23	t	Pre-mRNA-splicing factor SNT309	polymer	175	20741.5	1	UniProt (Q06091) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Synergistic to PRP19 mutation protein 309
24	u, i	Small nuclear ribonucleoprotein E	polymer	94	10385.1	2	UniProt (Q12330) Pfam (PF01423) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	snRNP-E
25	m, z	Small nuclear ribonucleoprotein Sm D1	polymer	146	16296.8	2	UniProt (Q02260) Pfam (PF01423) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Sm-D1
26	j, x	Small nuclear ribonucleoprotein G	polymer	77	8490.8	2	UniProt (P40204) Pfam (PF01423) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	snRNP-G
27	h, w	Small nuclear ribonucleoprotein F	polymer	86	9669.9	2	UniProt (P54999) Pfam (PF01423) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	snRNP-F
28	g, e	Small nuclear ribonucleoprotein Sm D2	polymer	110	12876.1	2	UniProt (Q06217) Pfam (PF01423) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Sm-D2
29	k, s	Small nuclear ribonucleoprotein-associated protein B	polymer	196	22427.0	2	UniProt (P40018) Pfam (PF01423) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	snRNP-B
30	l, y	Small nuclear ribonucleoprotein Sm D3	polymer	101	11240.1	2	UniProt (P43321) Pfam (PF01423) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	Sm-D3
31	r, p, q, o	Pre-mRNA-processing factor 19	polymer	503	56629.8	4	UniProt (P32523) Pfam (PF08606) In PDB	Saccharomyces cerevisiae S288c (Baker's yeast)	RING-type E3 ubiquitin transferase PRP19
32	A	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	1	Chemie (IHP)
33	C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
34	E, C	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
35	Q, R, T	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	41
	Total formula weight	2074070.8
Non-Polymers*	Number of molecules	14
	Total formula weight	1721.5
All*	Total formula weight	2075792.3

*Water molecules are not included.