6J26

Crystal structure of the branched-chain polyamine synthase from Thermococcus kodakarensis (Tk-BpsA) in complex with N4-bis(aminopropyl)spermidine and 5'-methylthioadenosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	N(4)-bis(aminopropyl)spermidine synthase	polymer	371	42445.9	2	UniProt (Q5JIZ3) Pfam (PF01861) In PDB	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	Branched-chain polyamine synthase A
2	A, B	5'-DEOXY-5'-METHYLTHIOADENOSINE	non-polymer		297.3	2	Chemie (MTA)
3	A, B	N~1~,N~1~,N~1~-tris(3-azaniumylpropyl)butane-1,4-diaminium	non-polymer		264.5	2	Chemie (B5L)
4	A	FE (III) ION	non-polymer		55.8	1	Chemie (FE)
5		water	water		18.0	204	Chemie (HOH)

Sequence modifications

A, B: 1 - 351 (UniProt: Q5JIZ3)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	84891.9
Non-Polymers*	Number of molecules	5
	Total formula weight	1179.5
All*	Total formula weight	86071.3

*Water molecules are not included.