6J1J

Crystal structure of HypX from Aquifex aeolicus, A392F-I419F variant in complex with Tetrahydrofolic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Hydrogenase regulation HoxX	polymer	582	67876.5	1	UniProt (O67224) Pfam (PF00551) Pfam (PF02911) Pfam (PF00378) In PDB	Aquifex aeolicus VF5
2	A	COENZYME A	non-polymer		767.5	1	Chemie (COA)
3	A	(6S)-5,6,7,8-TETRAHYDROFOLATE	non-polymer		445.4	1	Chemie (THG)
4	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	80	Chemie (HOH)

Sequence modifications

A: 1 - 562 (UniProt: O67224)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Phe 392	Ala 392	engineered mutation
Phe 419	Ile 419	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	67876.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1397.2
All*	Total formula weight	69273.7

*Water molecules are not included.