6J1H

Crystal structure of HypX from Aquifex aeolicus, Q15A-R131A-S194A-Q195A-N306A-R542A variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Hydrogenase regulation HoxX	polymer	582	67421.1	2	UniProt (O67224) Pfam (PF00551) Pfam (PF02911) Pfam (PF00378)	Aquifex aeolicus (strain VF5)
2	C, D (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	E, F (A, B)	water	water		18.0	76	Chemie (HOH)

Sequence modifications

A, B: 1 - 562 (UniProt: O67224)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 15	Gln 15	engineered mutation
Ala 131	Arg 131	engineered mutation
Ala 194	Ser 194	engineered mutation
Ala 195	Gln 195	engineered mutation
Ala 306	Asn 306	engineered mutation
Ala 542	Arg 542	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	134842.2
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	135026.4

*Water molecules are not included.