6J0G

Crystal structure of intracellular B30.2 domain of BTN3A3 mutant in complex with HMBPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Butyrophilin subfamily 3 member A3	polymer	221	25272.4	4	UniProt (O00478) Pfam (PF13765) Pfam (PF00622) In PDB	Homo sapiens (Human)
2	A, B, C, D	(2E)-4-hydroxy-3-methylbut-2-en-1-yl trihydrogen diphosphate	non-polymer		262.1	4	Chemie (H6P)
3		water	water		18.0	830	Chemie (HOH)

Sequence modifications

A, B, C, D: 298 - 485 (UniProt: O00478)

PDB	External Database	Details
Met 265	-	initiating methionine
Gly 266	-	expression tag
Ser 267	-	expression tag
Ser 268	-	expression tag
His 269	-	expression tag
His 270	-	expression tag
His 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag
Ser 275	-	expression tag
Ser 276	-	expression tag
Gly 277	-	expression tag
Leu 278	-	expression tag
Val 279	-	expression tag
Pro 280	-	expression tag
Arg 281	-	expression tag
Gly 282	-	expression tag
Ser 283	-	expression tag
His 284	-	expression tag
Met 285	-	expression tag
Glu 286	-	expression tag
Asn 287	-	expression tag
Leu 288	-	expression tag
Tyr 289	-	expression tag
Phe 290	-	expression tag
Gln 291	-	expression tag
Gly 292	-	expression tag
Ala 293	-	expression tag
Gly 294	-	expression tag
Ala 295	-	expression tag
Gly 296	-	expression tag
Ala 297	-	expression tag
His 351	Arg 381	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	101089.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1048.4
All*	Total formula weight	102138.0

*Water molecules are not included.