6IX9
The structure of LepI C52A in complex with SAM and leporin C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | O-methyltransferase lepI | polymer | 405 | 45459.6 | 2 | UniProt (B8NJH3) Pfam (PF00891) | Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) | Leporins biosynthesis protein I |
| 2 | C, F (A, B) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 2 | Chemie (SAM) | |||
| 3 | D (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 4 | E (B) | (6R,6aS,10S,10aR)-10-methyl-4-phenyl-6-[(1E)-prop-1-en-1-yl]-2,6,6a,7,8,9,10,10a-octahydro-1H-[2]benzopyrano[4,3-c]pyridin-1-one | non-polymer | 335.4 | 1 | Chemie (B0O) | |||
| 5 | G (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | H (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | I, J (A, B) | water | water | 18.0 | 546 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 387 (UniProt: B8NJH3)
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| Gly -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Ser -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Ala 1 | - | expression tag |
| Ala 52 | Cys 52 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 90919.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1282.9 | |
| All* | Total formula weight | 92202.1 |
