6ITC
Structure of a substrate engaged SecA-SecY protein translocation machine
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Protein translocase subunit SecA | polymer | 780 | 88916.2 | 1 | UniProt (P28366) Pfam (PF07517) Pfam (PF01043) Pfam (PF21090) Pfam (PF07516) In PDB | Bacillus subtilis (strain 168) | |
2 | Y | Protein translocase subunit SecY | polymer | 424 | 46768.3 | 1 | UniProt (A4IJK8) Pfam (PF00344) In PDB | Geobacillus thermodenitrificans (strain NG80-2) | |
3 | E | Protein translocase subunit SecE | polymer | 70 | 8249.6 | 1 | UniProt (A4IJH4) Pfam (PF00584) In PDB | Geobacillus thermodenitrificans (strain NG80-2) | |
4 | V | Nanobody | polymer | 116 | 12919.5 | 1 | Lama glama | ||
5 | B | Translocating peptide | polymer | 59 | 6024.6 | 1 | UniProt (by SIFTS) (P0A910) In PDB | Escherichia coli | |
6 | G | Green fluorescent protein | polymer | 238 | 26813.1 | 1 | UniProt (P42212) Pfam (PF01353) In PDB | Aequorea victoria (Jellyfish) | |
7 | C | Nanobody | polymer | 112 | 12368.7 | 1 | Lama glama | ||
8 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
9 | A | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 1 | Chemie (BEF) | |||
10 | A | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
11 | Y | (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE | non-polymer | 749.0 | 2 | Chemie (PGV) |
Sequence modifications
Y: 1 - 430 (UniProt: A4IJK8)
E: 1 - 60 (UniProt: A4IJH4)
G: 1 - 238 (UniProt: P42212)
PDB | External Database | Details |
---|---|---|
Cys 60 | Gly 60 | engineered mutation |
Thr 202 | Gln 202 | engineered mutation |
Gly 210 | Glu 204 | engineered mutation |
Gly 211 | Asn 205 | engineered mutation |
- | Val 206 | deletion |
- | Gly 207 | deletion |
- | Glu 208 | deletion |
- | Asp 209 | deletion |
- | Leu 210 | deletion |
- | Phe 211 | deletion |
Asn 213 | Arg 213 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 61 | - | expression tag |
Gly 62 | - | expression tag |
His 63 | - | expression tag |
His 64 | - | expression tag |
His 65 | - | expression tag |
His 66 | - | expression tag |
His 67 | - | expression tag |
His 68 | - | expression tag |
His 69 | - | expression tag |
His 70 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gys 66 | Ser 65 | chromophore |
Gys 66 | Tyr 66 | chromophore |
Gys 66 | Gly 67 | chromophore |
Arg 80 | Gln 80 | engineered mutation |
Ser 99 | Phe 99 | engineered mutation |
Thr 153 | Met 153 | engineered mutation |
Ala 163 | Val 163 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 7 |
Total formula weight | 202060.0 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 2015.5 | |
All* | Total formula weight | 204075.6 |