6ITC
Structure of a substrate engaged SecA-SecY protein translocation machine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein translocase subunit SecA | polymer | 780 | 88916.2 | 1 | UniProt (P28366) Pfam (PF07517) Pfam (PF01043) Pfam (PF21090) Pfam (PF07516) | Bacillus subtilis (strain 168) | |
| 2 | B (Y) | Protein translocase subunit SecY | polymer | 424 | 46768.3 | 1 | UniProt (A4IJK8) Pfam (PF00344) | Geobacillus thermodenitrificans (strain NG80-2) | |
| 3 | C (E) | Protein translocase subunit SecE | polymer | 70 | 8249.6 | 1 | UniProt (A4IJH4) Pfam (PF00584) | Geobacillus thermodenitrificans (strain NG80-2) | |
| 4 | D (V) | Nanobody | polymer | 116 | 12919.5 | 1 | Lama glama | ||
| 5 | E (B) | Translocating peptide | polymer | 59 | 6024.6 | 1 | UniProt (by SIFTS) (P0A910) | Escherichia coli | |
| 6 | F (G) | Green fluorescent protein | polymer | 238 | 26813.1 | 1 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria (Jellyfish) | |
| 7 | G (C) | Nanobody | polymer | 112 | 12368.7 | 1 | Lama glama | ||
| 8 | H (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 9 | I (A) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 1 | Chemie (BEF) | |||
| 10 | J (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 11 | K, L (Y) | (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE | non-polymer | 749.0 | 2 | Chemie (PGV) |
Sequence modifications
Y: 1 - 430 (UniProt: A4IJK8)
E: 1 - 60 (UniProt: A4IJH4)
G: 1 - 238 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Cys 60 | Gly 60 | engineered mutation |
| Thr 202 | Gln 202 | engineered mutation |
| Gly 210 | Glu 204 | engineered mutation |
| Gly 211 | Asn 205 | engineered mutation |
| - | Val 206 | deletion |
| - | Gly 207 | deletion |
| - | Glu 208 | deletion |
| - | Asp 209 | deletion |
| - | Leu 210 | deletion |
| - | Phe 211 | deletion |
| Asn 213 | Arg 213 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 61 | - | expression tag |
| Gly 62 | - | expression tag |
| His 63 | - | expression tag |
| His 64 | - | expression tag |
| His 65 | - | expression tag |
| His 66 | - | expression tag |
| His 67 | - | expression tag |
| His 68 | - | expression tag |
| His 69 | - | expression tag |
| His 70 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gys 66 | Ser 65 | chromophore |
| Gys 66 | Tyr 66 | chromophore |
| Gys 66 | Gly 67 | chromophore |
| Arg 80 | Gln 80 | engineered mutation |
| Ser 99 | Phe 99 | engineered mutation |
| Thr 153 | Met 153 | engineered mutation |
| Ala 163 | Val 163 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 202060.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 2015.5 | |
| All* | Total formula weight | 204075.6 |
