6ISR
structure of lipase mutant with Cys-His-Asp catalytic triad
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Lipase B | polymer | 321 | 33441.7 | 2 | UniProt (P41365) | Pseudozyma antarctica (Yeast) | CALB |
| 2 | C, F (A, B) | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) | |||
| 3 | D, G, H (A, B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 3 | Chemie (PEG) | |||
| 4 | E, I (A, B) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 2 | Chemie (PG4) | |||
| 5 | J, K (A, B) | water | water | 18.0 | 57 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 317 (UniProt: P41365)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ala 57 | Thr 82 | engineered mutation |
| Thr 89 | Ala 114 | engineered mutation |
| Val 104 | Trp 129 | engineered mutation |
| Cys 105 | Ser 130 | engineered mutation |
| Gly 149 | Val 174 | engineered mutation |
| Tyr 281 | Ala 306 | engineered mutation |
| Tyr 282 | Ala 307 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 66883.5 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 824.2 | |
| All* | Total formula weight | 67707.7 |
