6ISI
Structure of 9N-I DNA polymerase incorporation with 3'-CL in the active site
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA polymerase | polymer | 783 | 90939.8 | 1 | UniProt (Q56366) Pfam (PF03104) Pfam (PF00136) In PDB | Thermococcus sp. 9oN-7 | |
2 | C | DNA (5'-D(P*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*CP*C)-3') | polymer | 15 | 4545.9 | 1 | synthetic construct | ||
3 | D | DNA (5'-D(P*AP*CP*GP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3') | polymer | 18 | 5566.6 | 1 | synthetic construct | ||
4 | A | PYROPHOSPHATE | non-polymer | 178.0 | 1 | Chemie (PPV) | |||
5 | A, D | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
6 | A | 3-[2-[2-(2-azanylethoxy)ethoxy]ethoxy]propanoic acid | non-polymer | 221.3 | 1 | Chemie (B0U) | |||
7 | water | water | 18.0 | 21 | Chemie (HOH) |
Sequence modifications
A: 1 - 775 (UniProt: Q56366)
PDB | External Database | Details |
---|---|---|
Ala 141 | Asp 141 | engineered mutation |
Ala 143 | Glu 143 | engineered mutation |
Leu 485 | Ala 485 | engineered mutation |
Leu 776 | - | expression tag |
Glu 777 | - | expression tag |
His 778 | - | expression tag |
His 779 | - | expression tag |
His 780 | - | expression tag |
His 781 | - | expression tag |
His 782 | - | expression tag |
His 783 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 101052.4 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 559.5 | |
All* | Total formula weight | 101611.9 |