6ISH

Structure of 9N-I DNA polymerase incorporation with 3'-AL in the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase	polymer	783	90939.8	1	UniProt (Q56366) Pfam (PF03104) Pfam (PF00136) In PDB	Thermococcus sp. 9oN-7
2	C	DNA (5'-D(P*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*CP*(DAL))-3')	polymer	14	4256.8	1		synthetic construct
3	D	DNA (5'-D(*AP*CP*TP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*C)-3')	polymer	17	5212.4	1		synthetic construct
4	A	PYROPHOSPHATE	non-polymer		178.0	1	Chemie (PPV)
5	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	C	[(2R,3S,5R)-5-(6-aminopurin-9-yl)-2-(phosphonooxymethyl)oxolan-3-yl] 3-(2-ethoxyethoxy)propanoate	non-polymer		475.4	1	Chemie (B9X)
7		water	water		18.0	22	Chemie (HOH)

Sequence modifications

A: 1 - 775 (UniProt: Q56366)

PDB	External Database	Details
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation
Leu 485	Ala 485	engineered mutation
Leu 776	-	expression tag
Glu 777	-	expression tag
His 778	-	expression tag
His 779	-	expression tag
His 780	-	expression tag
His 781	-	expression tag
His 782	-	expression tag
His 783	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	100409.0
Non-Polymers*	Number of molecules	4
	Total formula weight	733.5
All*	Total formula weight	101142.5

*Water molecules are not included.