6ISG

Structure of 9N-I DNA polymerase incorporation with dG in the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase	polymer	783	90939.8	1	UniProt (Q56366) Pfam (PF03104) Pfam (PF00136)	Thermococcus sp. 9oN-7
2	B (C)	DNA (5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*CP*G)-3')	polymer	15	4586.0	1		synthetic construct
3	C (D)	DNA (5'-D(*GP*AP*CP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*G)-3')	polymer	18	5566.6	1		synthetic construct
4	D (A)	PYROPHOSPHATE	non-polymer		178.0	1	Chemie (PPV)
5	E, F, G, H, I (A, D)	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)
6	J, K, L (A, C, D)	water	water		18.0	30	Chemie (HOH)

Sequence modifications

A: 1 - 775 (UniProt: Q56366)

PDB	External Database	Details
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation
Leu 485	Ala 485	engineered mutation
Leu 776	-	expression tag
Glu 777	-	expression tag
His 778	-	expression tag
His 779	-	expression tag
His 780	-	expression tag
His 781	-	expression tag
His 782	-	expression tag
His 783	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	101092.4
Non-Polymers*	Number of molecules	6
	Total formula weight	378.4
All*	Total formula weight	101470.8

*Water molecules are not included.