6IS7
Structure of 9N-I DNA polymerase incorporation with dA in the active site
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA polymerase | polymer | 783 | 90939.8 | 2 | UniProt (Q56366) Pfam (PF03104) Pfam (PF00136) In PDB | Thermococcus sp. 9oN-7 | |
2 | C, E, K, L | DNA (5'-D(P*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*CP*A)-3') | polymer | 15 | 4570.0 | 4 | synthetic construct | ||
3 | D, F, I | DNA (5'-D(*AP*CP*TP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3') | polymer | 18 | 5541.6 | 3 | synthetic construct | ||
4 | A, B | CALCIUM ION | non-polymer | 40.1 | 7 | Chemie (CA) | |||
5 | water | water | 18.0 | 212 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 775 (UniProt: Q56366)
PDB | External Database | Details |
---|---|---|
Ala 141 | Asp 141 | engineered mutation |
Ala 143 | Glu 143 | engineered mutation |
Leu 485 | Ala 485 | engineered mutation |
Leu 776 | - | expression tag |
Glu 777 | - | expression tag |
His 778 | - | expression tag |
His 779 | - | expression tag |
His 780 | - | expression tag |
His 781 | - | expression tag |
His 782 | - | expression tag |
His 783 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 9 |
Total formula weight | 216784.3 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 280.5 | |
All* | Total formula weight | 217064.9 |