6IS7

Structure of 9N-I DNA polymerase incorporation with dA in the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA polymerase	polymer	783	90939.8	2	UniProt (Q56366) Pfam (PF03104) Pfam (PF00136) In PDB	Thermococcus sp. 9oN-7
2	C, E, K, L	DNA (5'-D(P*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*CP*A)-3')	polymer	15	4570.0	4		synthetic construct
3	D, F, I	DNA (5'-D(*AP*CP*TP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3')	polymer	18	5541.6	3		synthetic construct
4	A, B	CALCIUM ION	non-polymer		40.1	7	Chemie (CA)
5		water	water		18.0	212	Chemie (HOH)

Sequence modifications

A, B: 1 - 775 (UniProt: Q56366)

PDB	External Database	Details
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation
Leu 485	Ala 485	engineered mutation
Leu 776	-	expression tag
Glu 777	-	expression tag
His 778	-	expression tag
His 779	-	expression tag
His 780	-	expression tag
His 781	-	expression tag
His 782	-	expression tag
His 783	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	216784.3
Non-Polymers*	Number of molecules	7
	Total formula weight	280.5
All*	Total formula weight	217064.9

*Water molecules are not included.