6IQY

High resolution structure of bilirubin oxidase from Myrothecium verrucaria - M467Q mutant, anaerobically prepared

Replaces:  5ZD7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bilirubin oxidase	polymer	538	60545.2	2	UniProt (Q12737)	Myrothecium verrucaria (Myrothecium leaf spot and pod blight fungus)
2	C, D (C, D)	alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G62182OO)
3	E, F, G, H, O... (A, B)	COPPER (II) ION	non-polymer		63.5	8	Chemie (CU)
4	I, S (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5	J, T (A, B)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
6	K, L, M, U, V... (A, B)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
7	N, Y (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
8	AA, Z (B, A)	water	water		18.0	1481	Chemie (HOH)

Sequence modifications

A, B: 1 - 534 (UniProt: Q12737)

PDB	External Database	Details
Tyr -3	-	expression tag
Val -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Gln 467	Met 505	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	121090.5
Branched	Number of molecules	2
	Total formula weight	1173.1
Non-Polymers*	Number of molecules	21
	Total formula weight	1793.7
All*	Total formula weight	124057.2

*Water molecules are not included.