6IK7

Crystal structure of tomato beta-galactosidase (TBG) 4 in complex with beta-1,3-galactobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	Beta-galactosidase	polymer	718	79726.9	2	UniProt (O81100) Pfam (PF01301) Pfam (PF17834) Pfam (PF21467) In PDB	Solanum lycopersicum (Tomato)
2	C, E	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
3	D, F	beta-D-galactopyranose-(1-3)-beta-D-galactopyranose	branched		342.3	2	In PDB GlyTouCan (G95894VW)
4	B, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5		water	water		18.0	109	Chemie (HOH)

Sequence modifications

B, A: 24 - 724 (UniProt: O81100)

PDB	External Database	Details
Glu 18	-	expression tag
Ala 19	-	expression tag
Glu 20	-	expression tag
Ala 21	-	expression tag
Glu 22	-	expression tag
Phe 23	-	expression tag
Ala 181	Glu 181	engineered mutation
Ser 725	-	expression tag
Ala 726	-	expression tag
Ala 727	-	expression tag
Ala 728	-	expression tag
Ala 729	-	expression tag
Ser 730	-	expression tag
Phe 731	-	expression tag
Leu 732	-	expression tag
Glu 733	-	expression tag
Gln 734	-	expression tag
Lys 735	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	159453.8
Branched	Number of molecules	4
	Total formula weight	1533.4
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	161429.6

*Water molecules are not included.