6IFK
Cryo-EM structure of type III-A Csm-CTR1 complex, AMPPNP bound
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Type III-A CRISPR-associated protein Csm1 | polymer | 758 | 86976.7 | 1 | UniProt (A0A2U2M0F3) | Streptococcus thermophilus ND03 | |
| 2 | B, C (D, C) | Type III-A CRISPR-associated protein Csm2 | polymer | 126 | 14848.1 | 2 | UniProt (A0A2U2M049) | Streptococcus thermophilus ND03 | |
| 3 | D, E, F (G, F, E) | Type III-A CRISPR-associated RAMP protein Csm3 | polymer | 220 | 24584.9 | 3 | UniProt (A0A2U2M035) | Streptococcus thermophilus ND03 | |
| 4 | G (B) | Type III-A CRISPR-associated RAMP protein Csm4 | polymer | 299 | 33829.0 | 1 | UniProt (A0A2U2M037) | Streptococcus thermophilus ND03 | |
| 5 | H (H) | Type III-A CRISPR-associated RAMP protein Csm5 | polymer | 357 | 41102.1 | 1 | UniProt (A0A2U2M038) | Streptococcus thermophilus ND03 | |
| 6 | I (N) | crRNA | polymer | 34 | 10780.4 | 1 | Streptococcus thermophilus ND03 | ||
| 7 | J (J) | CTR1 | polymer | 42 | 13701.2 | 1 | Streptococcus thermophilus ND03 | ||
| 8 | K, L (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) | |||
| 9 | M, N (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 10 | O (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
A: 1 - 758 (UniProt: A0A2U2M0F3)
G, F, E: 1 - 220 (UniProt: A0A2U2M035)
| PDB | External Database | Details |
|---|---|---|
| Asn 16 | Asp 16 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asn 33 | Asp 33 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 289840.4 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1126.4 | |
| All* | Total formula weight | 290966.8 |
