6IFF

Crystal structure of M1 zinc metallopeptidase E323A mutant from Deinococcus radiodurans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Zinc metalloprotease	polymer	474	51547.8	2	UniProt (Q9RVZ5) Pfam (PF17900) Pfam (PF01433)	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	M1 zinc metallopeptidase
2	C, F (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	D, G (A, B)	TYROSINE	non-polymer		181.2	2	Chemie (TYR)
4	E, H (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	I, J (A, B)	water	water		18.0	436	Chemie (HOH)

Sequence modifications

A, B: 36 - 472 (UniProt: Q9RVZ5)

PDB	External Database	Details
Met -1	-	initiating methionine
Ala 0	-	expression tag
Ser 1	-	expression tag
Trp 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
Pro 5	-	expression tag
Gln 6	-	expression tag
Phe 7	-	expression tag
Glu 8	-	expression tag
Lys 9	-	expression tag
Gly 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
Ser 19	-	expression tag
Ser 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
Gly 23	-	expression tag
Gly 24	-	expression tag
Gly 25	-	expression tag
Gly 26	-	expression tag
Gly 27	-	expression tag
Glu 28	-	expression tag
Asn 29	-	expression tag
Leu 30	-	expression tag
Tyr 31	-	expression tag
Phe 32	-	expression tag
Gln 33	-	expression tag
Gly 34	-	expression tag
Ser 35	-	expression tag
Ala 323	Glu 323	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	103095.6
Non-Polymers*	Number of molecules	6
	Total formula weight	539.2
All*	Total formula weight	103634.8

*Water molecules are not included.