6I95

R2-like ligand-binding oxidase G68L mutant with anaerobically reconstituted Mn/Fe cofactor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ribonucleotide reductase small subunit	polymer	316	37018.9	1	UniProt (Q5KW80) Pfam (PF00268) In PDB	Geobacillus kaustophilus (strain HTA426)
2	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
3	A	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
4	A	OCTANOIC ACID (CAPRYLIC ACID)	non-polymer		144.2	1	Chemie (OCA)
5		water	water		18.0	102	Chemie (HOH)

Sequence modifications

A: 1 - 302 (UniProt: Q5KW80)

PDB	External Database	Details
Met -13	-	initiating methionine
Ala -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Leu 68	Gly 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37018.9
Non-Polymers*	Number of molecules	4
	Total formula weight	310.8
All*	Total formula weight	37329.7

*Water molecules are not included.