6I8A

The crystal structure of the Pol2 catalytic domain of DNA polymerase epsilon carrying a P301R substitution.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA polymerase epsilon catalytic subunit A	polymer	1190	137134.6	2	UniProt (P21951) Pfam (PF03104) Pfam (PF00136) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	DNA polymerase II subunit A
2	P, C	Primer DNA	polymer	11	3293.2	2		synthetic construct
3	T, D	Template DNA	polymer	16	4889.2	2		synthetic construct
4	A, B	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	2	Chemie (DTP)
5	A, B	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	A, B	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
7		water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, B: 1 - 1185 (UniProt: P21951)

PDB	External Database	Details
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Arg 301	Pro 301	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	290633.8
Non-Polymers*	Number of molecules	8
	Total formula weight	1254.4
All*	Total formula weight	291888.2

*Water molecules are not included.