6I8A
The crystal structure of the Pol2 catalytic domain of DNA polymerase epsilon carrying a P301R substitution.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, B) | DNA polymerase epsilon catalytic subunit A | polymer | 1190 | 137134.6 | 2 | UniProt (P21951) Pfam (PF03104) Pfam (PF22634) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | DNA polymerase II subunit A |
| 2 | B, E (P, C) | Primer DNA | polymer | 11 | 3293.2 | 2 | synthetic construct | ||
| 3 | C, F (T, D) | Template DNA | polymer | 16 | 4889.2 | 2 | synthetic construct | ||
| 4 | G, K (A, B) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 2 | Chemie (DTP) | |||
| 5 | H, I, L, M (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 6 | J, N (A, B) | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) | |||
| 7 | O, P, Q (A, T, B) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 1185 (UniProt: P21951)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| Arg 301 | Pro 301 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 290633.8 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1254.4 | |
| All* | Total formula weight | 291888.2 |
