6I53
Cryo-EM structure of the human synaptic alpha1-beta3-gamma2 GABAA receptor in complex with Megabody38 in a lipid nanodisc
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (E, B) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 473 | 54444.6 | 2 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit beta-3 |
| 2 | B, E (A, D) | Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 464 | 52916.6 | 2 | UniProt (P14867) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit alpha-1 |
| 3 | D (C) | Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 495 | 56922.1 | 1 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit gamma-2 |
| 4 | F (G) | Megabody38 | polymer | 123 | 13462.9 | 1 | Lama glama | ||
| 5 | G, M (F, M) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 2 | In PDB GlyTouCan (G22768VO) | |||
| 6 | H, K, L (H, K, L) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 7 | I (I) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G55220VL) | |||
| 8 | J (J) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G09724ZC) | |||
| 9 | N, P, R (E, A, D) | (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate | non-polymer | 760.1 | 3 | Chemie (POV) | |||
| 10 | O, Q (A, D) | [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate | non-polymer | 746.6 | 2 | Chemie (PIO) |
Sequence modifications
A, D: -34 - 429 (UniProt: P14867)
C: -38 - 436 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Lys -33 | Arg 2 | conflict |
| Tyr -25 | Cys 10 | conflict |
| Thr -20 | Ile 15 | conflict |
| Phe -18 | Leu 17 | conflict |
| Asp -7 | - | insertion |
| Tyr -6 | - | insertion |
| Lys -5 | - | insertion |
| Asp -4 | - | insertion |
| Asp -3 | - | insertion |
| Asp -2 | - | insertion |
| Asp -1 | - | insertion |
| Lys 0 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Gly 437 | - | expression tag |
| Gly 438 | - | expression tag |
| Ser 439 | - | expression tag |
| Gly 440 | - | expression tag |
| Gly 441 | - | expression tag |
| Ser 442 | - | expression tag |
| Gly 443 | - | expression tag |
| Gly 444 | - | expression tag |
| Ser 445 | - | expression tag |
| Gly 446 | - | expression tag |
| Lys 447 | - | expression tag |
| Thr 448 | - | expression tag |
| Glu 449 | - | expression tag |
| Thr 450 | - | expression tag |
| Ser 451 | - | expression tag |
| Gln 452 | - | expression tag |
| Val 453 | - | expression tag |
| Ala 454 | - | expression tag |
| Pro 455 | - | expression tag |
| Ala 456 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 285107.3 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 5402.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 3773.4 | |
| All* | Total formula weight | 294283.6 |
