6I2M

Crystal structure of vaccinia virus protein A55 BTB-Back domain in complex with human Cullin-3 N-terminus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Kelch repeat and BTB domain-containing protein A55	polymer	259	29961.0	1	UniProt (P24768) Pfam (PF00651) Pfam (PF07707)	Vaccinia virus WR
2	B (B)	Cullin-3	polymer	388	45724.0	1	UniProt (Q13618) Pfam (PF00888)	Homo sapiens (Human)	CUL-3

Sequence modifications

A: 1 - 250 (UniProt: P24768)

PDB	External Database	Details
Gly 251	-	expression tag
Ser 252	-	expression tag
Lys 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag

B: 23 - 388 (UniProt: Q13618)

PDB	External Database	Details
Arg 342	Ile 342	engineered mutation
Asp 346	Leu 346	engineered mutation
Ala 389	-	expression tag
Glu 390	-	expression tag
Asn 391	-	expression tag
Leu 392	-	expression tag
Tyr 393	-	expression tag
Phe 394	-	expression tag
Gln 395	-	expression tag
Ser 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
Asp 403	-	expression tag
Tyr 404	-	expression tag
Lys 405	-	expression tag
Asp 406	-	expression tag
Asp 407	-	expression tag
Asp 408	-	expression tag
Asp 409	-	expression tag
Lys 410	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	75684.9
All*	Total formula weight	75684.9

*Water molecules are not included.